FASTQ + Emoji = FASTQE 🤔
Compute quality stats for FASTQ files and print those stats as emoji... for some reason.
Install
Python 3 required. Tested on Linux and Mac.
Windows partially suported and requires Windows Terminal.
pip install fastqe
Usage
See the README for more options.fastqe [--bin] [--min] [--max] [FASTQ_FILE ...]
Mapping & Scale Bar
See README for more detail on the mapping from phred scores to emoji:
🚫❌👺💔🙅👾👿💀👻🙈🙉🙊🐵😿😾🙀💣🔥😡💩⚠️
😀😅😏😊😙😗😚😃😘😆😄😋😄😝😛😜😉😁😄😎😍
Scores can also be binned:
| Bin | Emoji |
|---|---|
| N | 🚫 |
| 2-9 | 💀 |
| 10–19 | 💩 |
| 20–24 | ⚠️ |
| 25–29 | 😄 |
| 30–34 | 😆 |
| 35–39 | 😎 |
| ≥ 40 | 😍 |
Biomojify
A companion program, biomojify, will convert sequences rather than summarise them:
▶️ SEQUENCE_1
🥑🍅🥑🍇🍅🌽🥑🍇🍅🥑🌽🍇🍅🥑🍇🍅🌽🍇🥑🍅🍇🌽🍅🥑🍇🌽🍅🥑🍇🍅🥑🍇🍇
🍇🍇🍇🍇🌽🍅🍇🥑🍅🍇🥑🍅🍇🍅🥑🍇🌽🍅🌽🍇🥑🍅🌽🍇🍅🥑🌽🍇🍅🥑🌽🍇🍅
🥑🌽🍇🌽🍅🍇🥑🍇🍅🌽🥑🍇🍅🍇🌽🥑🌽🍇🍅🥑🌽🍇🌽🍅🍇🌽🥑🍅🍇🌽🌽❓🍅
🥑🍇❓🌽🍅🥑🥑🥑🥑🍇❓🌽🍅🥑❓🍇🌽🍅🥑🍇🌽❓🍅🥑❓🍇🌽🍅🍇🥑🌽🍅❓
🥑🍇❓🍅🍇🥑🌽🍅🍇🌽❓🍅🌽🍇🍅🌽🍇❓🥑🍅🌽🥑🍅🍇🍅🥑🌽🍇🍅🥑🍇🌽🍇
🥑🍇🌽🍅🍅🍅🍅🍅🍅🍅🥑🍇🍅🍇🍅🥑🌽🍇🍅🥑🍇🍅🥑🌽🍅🥑🌽🌽🌽🌽🌽🌽🌽
🌽🌽🍇🍅🥑🌽🍇🍅🥑🌽🍇🍅🥑🌽🍇🍅🌽🥑🌽🍇🍅🍇🌽🍅🍇🥑🌽🍅❓❓❓🥑🌽
🍇🥑🍅🌽🍇🍅🥑🍇🍅🥑🍇🌽🍅🍇🥑🌽🍅🍇🥑🍅🍇🌽🍅
See that project page for more information: https://github.com/fastqe/biomojify
Feedback
Submit bugs, enhancements and feedback via GitHub.
Contribute
There is lots of room for improvement in this tool. The majority of the code has been
written in the hours (and minutes) prior to presentations, so its almost guaranteed to be suboptimal.
Pull requests welcome on GitHub.
Development of FASTQE continues, and you can get involved by talking to us on Gitter or use the Open Chat button below.